Precisely defining disease variant effects in CRISPR-edited single...

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2025-07-24 02:28:31 -

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Precisely defining disease variant effects in CRISPR-edited single cells

Nature, Published online: 23 July 2025; doi:10.1038/s41586-025-09313-3A plate-based assay called CRAFTseq has been developed that uses multi-omic single-cell RNA sequencing and direct genotyping of CRISPR edits to test the functional effects of genetic variants on cell state and function.

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